ImagingLive-cell bioimaging
Long-duration confocal and two-photon imaging without bleaching, photodamage or autofluorescence interference.
Light that
doesn't fade.
Cytoglow's UCNP labeling technology pairs highly luminescent upconversion nanoparticles with robust instruments, software, and technical support — bringing powerful performance to research, life-science, and diagnostic applications.
0%
photobleaching
<10 nm
particle diameter
~10 nm
emission FWHM
10+
spectral channels
The Technology
Anti-Stokes emission,
engineered to last.
Cytoglow's UCNP labeling technology is built on photon upconversion: a near-infrared 980 nm excitation source drives emission at shorter visible wavelengths — the inverse of conventional fluorophores. The anti-Stokes process avoids interference from biological autofluorescence and produces exceptionally clear signals, frame after frame.
Zero autofluorescence
NIR excitation sits well below the absorption bands of biological samples — assays and images stay completely free of native tissue autofluorescence, delivering high signal-to-noise ratios from the first frame.
No photobleaching
Unlike organic dyes or quantum dots, lanthanide-doped UCNPs do not degrade under continuous excitation. Image the same field for hours — or repeat readouts for weeks — without measurable signal loss.
Deep tissue penetration
NIR excitation wavelengths travel substantially deeper into biological tissue than UV or visible light, opening up live-animal, thick-section, and intravital imaging workflows.
Applications
From the bench
to the clinic.
CytoGlow particles slot into existing fluorescence workflows with off-the-shelf 980 nm laser modules — no custom optics required.
Imaging
ImagingMultiplexed Immunofluorescence Imaging
Stain dozens of markers in a single section. Narrow ~10 nm emission lines unlock high-plex panels without spectral overlap or repeated round-stripping cycles.
In situSpatial omics
Profile genes, transcripts, and proteins directly within FFPE tissue. EverGlow™ enables high-plex in-situ molecular mapping that preserves the cell-neighborhood interactions defining each cell's functional state.
EverGlow™ Series
EverGlow™ — a particle
for every channel.
EverGlow™ is Cytoglow's flagship line of photobleach-resistant upconversion fluorophores. Four narrow-band emitters built from rare-earth lanthanide chemistry, all excited at 980 nm and shipped surface-functionalized with carboxyl, amine, or streptavidin conjugation.
EverGlow™ in vial
One excitation laser. The full visible spectrum.
λex = 980 nm
λem = 475 nm
EverGlow™ 475
Cerulean
NaYF₄:Yb,Tm
Core ⌀
25 ± 2 nm
Surface
-COOH / -NH₂
λem = 540 nm
EverGlow™ 540
Spring
NaYF₄:Yb,Er
Core ⌀
25 ± 2 nm
Surface
-COOH / -NH₂
λem = 660 nm
EverGlow™ 660
Ember
NaYF₄:Yb,Er
Core ⌀
25 ± 2 nm
Surface
-COOH / -NH₂
λem = 800 nm
EverGlow™ 800
Twilight
NaYF₄:Yb,Tm
Core ⌀
25 ± 2 nm
Surface
-COOH / -NH₂
Need a custom emission line, surface chemistry, or bulk volume? Talk to our chemists →
Founded
2024
Spun out of a university nono-material lab.
In-house manufacturing
Gram-scale UCNP synthesis with batch-to-batch QC by TEM, DLS and PL spectroscopy.
About CytoGlow
The worldwide leader in
upconversion nanomaterials.
Cytoglow LLC focuses on the R&D, production, and application of magneto-optical nanoparticles. We are the worldwide leading supplier of upconversion nanoparticle material — photobleach-resistant fluorescence dyes for biomedical applications, widely used in multiplexed immunofluorescence.
Beyond particles, Cytoglow specializes in developing and manufacturing innovative diagnostic tests, assay components, and instruments. Trusted by customers worldwide, we also provide assay development and contract manufacturing services for the IVD and life-science industries.
Built on 20 years of scientific R&D, our product matrix spans three core domains — and we continue to explore cross-disciplinary applications of nanomaterials across biomedicine, intelligent sensing, and new energy.
Fluorescence dyes
Spatial omics
Nano-sensors
Innovation/Intelligence/Excellence
Cytoglow: Innovation Illuminates the Future.
20+
Years research
8
Patents pending
3
Continents shipped
Get in touch
Request a sample,
or a conversation.
We respond within two business days with availability, spec sheets and a quote. Custom surface chemistry and bulk pricing on request.